General Protocols

Cell Culture in AIM System

  • Gel preparation →  Video
  • Gel filling → Video
  • Coating the media channels
  • Media channel hydration  →  Video
  • Cell seeding
  • Media replacement → Video
  • Cell staining
  • Troubleshooting
  • Reagent setup

Downloads

General Protocol v5.4 →
Quick Start Guide

Seeding Cells in Hydrogel

Application of Flow

  • Interstitial Flow
  • Shear Flow
  • Maintaining concentration gradients

Downloads

Application of Flow v3.3 →

 

Holder

  • Chip assembly
  • Chip removal
  • Humidity control

Downloads

Holder HOL-2 Handling Protocol v2.0 →
Quick Start Guide HOL-1 v1.1 →

Luer Connectors

  • Attaching AIM luer connectors to chips
  • ​Using Inlet Seals to seal off gel inlets to prevent media outflow

Downloads

LUC-1 Connector Handling Protocol v5.5 →
Quick Start Guide LUC-1 v1.2 →