General Protocols

General Protocols

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Cell Chip Culture

  • Gel preparation →  Video
  • Gel filling → Video
  • Coating the media channels
  • Media channel hydration  →  Video
  • Cell seeding
  • Media replacement → Video
  • Cell staining
  • Troubleshooting
  • Reagent setup

Downloads

General Protocol v5.4 →
Quick Start Guide DAX-1 v1.3 →

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Seeding Cells in Hydrogel

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Application of Flow

  • Interstitial Flow
  • Shear Flow
  • Maintaining concentration gradients

Downloads

Application of Flow v3.3 →

 

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Holder

  • Chip assembly
  • Chip removal
  • Humidity control

Downloads

Holder HOL-1 Handling Protocol v4.0 →
Quick Start Guide HOL-1 v1.1 →

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Luer Connectors

  • Attaching AIM luer connectors to chips
  • ​Using Inlet Seals to seal off gel inlets to prevent media outflow

Downloads

LUC-1 Connector Handling Protocol v5.4 →
Quick Start Guide LUC-1 v5.2 →