How to Handle Matrigel for 3D Cell Migration Assays

How to Handle Matrigel for 3D Cell Migration Assays

OverviewA researcher from China has been looking for an in vitro platform to run cancer cell migration assays. He found that AIM 3D Cell Culture Chips are suitable for his application as the chips offer useful features such as the ability to monitor live cell...
Tips and Tricks for Changing Medium

Tips and Tricks for Changing Medium

We have designed the ports for rapid medium exchange without the risk of over-aspiration.   Use an aspirator instead of a pipette to remove medium from the ports more efficiently. The cells in the channels will not be disturbed during medium removal due to the...
Tips and Tricks for Staining Cells in AIM Chips

Tips and Tricks for Staining Cells in AIM Chips

AIM chips are compatible with various imaging techniques, including fluorescence microscopy. In order to visualize the cells with fluorescence microscopy, you can use fluorescence-labelled cells (such as GFP-tagged cells) or stain the cells with fluorophore. Here are...
Tips and Tricks for Seeding Cells in 3D Gel Channels

Tips and Tricks for Seeding Cells in 3D Gel Channels

One of the benefits of AIM chips is the ability to seed cells within a 3D hydrogel. This may sound challenging at first but it can be as simple as hydrogel filling once you learn how.   Always take the osmolarity of the final hydrogel solution into consideration...
Tips and Tricks for Preparing Collagen I Gel

Tips and Tricks for Preparing Collagen I Gel

We understand that the preparation of collagen I gel can be daunting to new users. Here are some tips and tricks to help you get better in preparing collagen I gel.   Good pipetting skills and practices help minimize pipetting errors and reduce the formation of...