Spheroid Dispersion

Cancer spheroid dispersion assays are used to model the epithelial mesenchymal transition (EMT) that occurs in the early stages of cancer metastasis.

Pre-formed cancer spheroids can be seeded in the 3D hydrogel region of AIM chips and co-cultured with secondary cell types (such as endothelial cells) to mimic the tumor microenvironment more accurately.

Cancer cell migration rates and other details of the dispersion process can be monitored closely in real time to gain insights into metastatic events.

Spheroid Dispersion

Protocols

The idenTx chip design is suitable for cancer spheroid dispersion assays; however, the detailed protocols for the AIM chips are currently being developed. Please refer to (1) in  the publication list below for protocols using PDMS versions of the chip.

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Key References

  1. Retina-on-chip: engineering functional in vitro models of the human retina using organ-on-chip technology. Oosten, E. M., & ávan der Meer, A. D. (2025). Lab on a Chip
  2. Pericytes Promote More Vascularization than Stromal Cells via an Interleukin‐6‐Dependent Mechanism in Microfluidic Chips. Gonzalez‐Rubio, J., Kubiza, H., Xu, Y., Koenigs‐Werner, H., Schmitz, M. S., Schedel, M., … & Thiebes, A. L. (2025). Advanced Science, 2408131
  3. Assessing personalized responses to anti-PD-1 treatment using patient-derived lung tumor-on-chip. Veith, I., Nurmik, M., Mencattini, A., Damei, I., Lansche, C., Brosseau, S., … & Parrini, M. C. (2024). Cell Reports Medicine, 5(5)
  4. Analysis of EMT induction in a non-invasive breast cancer cell line by mesenchymal stem cell supernatant: Study of 2D and 3D microfluidic based aggregate. Azadi, S., Torkashvand, E., Mohammadi, E., & Tafazzoli-Shadpour, M. (2023). Life Sciences, 320, 121545
  5. Jagged1 intracellular domain/SMAD3 complex transcriptionally regulates TWIST1 to drive glioma invasion. Kim, J. Y., Hong, N., Park, S., Ham, S. W., Kim, E. J., Kim, S. O., … & Kim, H. (2023). Cell Death & Disease, 14(12), 822
  6. Ex vivo treatment in high grade serous ovarian cancer demonstrates the benefit of EZH2 inhibition in combination with standard therapy. Vo, H. V., Zeng, Q., Barbie, D. A., Gokhale, P. C., Adams, E., Paweletz, C. P., & Ivanova, E. (2022).  Cancer Research, 82(12_Supplement), 3264-3264
  7. Radiobiological effects of wound fluid on breast cancer cell lines and human-derived tumor spheroids in 2D and microfluidic culture. Jeibouei, S., Hojat, A., Mostafavi, E., Aref, A. R., Kalbasi, A., Niazi, V., … & Zali, H. (2022).  Scientific Reports, 12(1), 7668
  8. Evaluation of Tumor Response to Adjuvant Treatments using an Ex Vivo Culture of Breast Carcinoma Spheroids in a Microfluidic Device. Es, H. A., Aref, A. R., Granpayeh, L., Ebrahimi, M., & Baharvand, H. (2021). MedRxiv, 2021-05
  9. Pirfenidone Reduces Epithelial Mesenchymal Transition and Spheroid Formation in Breast Carcinoma through Targeting Cancer-Associated Fibroblasts. Es, H. A., Cox, T. R., Sarafraz-Yazdi, E., Thiery, J. P., & Warkiani, M. E. (2021). Cancers, 13(20), 5118
  10. Rapid spheroid assays in a 3-dimensional cell culture chip. Teh, J. L., Abdul Rahman, S. F., Domnic, G., Satiyasilan, L., Chear, N. J. Y., Singh, D., & Mohana-Kumaran, N. (2021).  BMC research notes, 14, 1-6

All publications related to Spheroid Dispersion, can be found here.

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